Expression of Genes Affecting Skin Coloration and Sugar Accumulation in Apple Fruits at Ripening Stages in High Temperatures
نویسندگان
چکیده
High temperature is one of the most important environmental factors that affect the successful cultivation of apple (Malus × domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation by high temperature treatment was investigated in the fruits of “Fuji” apple at different developmental stages in different temperature conditions through realtime PCR. In the initial ripening stages in fruits, there was high expression of genes associated with fruit ripening, flavonoid compound accumulation, and coloration in high temperatures. Expression of phenylalanine ammonia-lyase, chalcone synthase, flavone 3-hydrogenase, and malate dehydrogenase genes increased gradually in initial ripening stages, while sharply reduced at 24 hr after treatment. In addition, there was no significant difference in the expression of all temperature treatments in late ripening stages. In the further work, investigation of expression levels of various genes could be conducted in the level of transcriptomes from fruits at the early stages to get meaningful information of ripening metabolism in apples in high temperatures.
منابع مشابه
Proteomics and SSH Analyses of ALA-Promoted Fruit Coloration and Evidence for the Involvement of a MADS-Box Gene, MdMADS1
Skin color is a key quality attribute of fruits and how to improve fruit coloration has long been a major concern. 5-Aminolevulinic acid (ALA), a natural plant growth regulator, can significantly increase anthocyanin accumulation in fruit skin and therefore effectively improve coloration of many fruits, including apple. However, the molecular mechanism how ALA stimulates anthocyanin accumulatio...
متن کاملIndependence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA
MdMYB10 gene expression results in accumulation of anthocyanin in many tissues including flesh of applefruit. The MdMYB1 and MdMYBA genes are close homologues to MdMYB10 gene and both are responsiblefor red color phenotype in apple fruit skin. In the current study, an apple genome sequence draft analysisindicated that these three genes are located in a unique contig. Further a...
متن کاملPost-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera
Anthocyanins, flavan-3-ols, and flavonols are the three major classes of flavonoid compounds found in grape berry tissues. Several viticultural practices increase flavonoid content in the fruit, but the underlying genetic mechanisms responsible for these changes have not been completely deciphered. The impact of post-veraison sunlight exposure on anthocyanin and flavonol accumulation in grape b...
متن کاملSalinity induces carbohydrate accumulation and sugar-regulated starch biosynthetic genes in tomato (Solanum lycopersicum L. cv. ‘Micro-Tom’) fruits in an ABA- and osmotic stress-independent manner
Salinity stress enhances sugar accumulation in tomato (Solanum lycopersicum) fruits. To elucidate the mechanisms underlying this phenomenon, the transport of carbohydrates into tomato fruits and the regulation of starch synthesis during fruit development in tomato plants cv. 'Micro-Tom' exposed to high levels of salinity stress were examined. Growth with 160 mM NaCl doubled starch accumulation ...
متن کاملIdentification of Differentially Expressed Genes Associated with Apple Fruit Ripening and Softening by Suppression Subtractive Hybridization
Apple is one of the most economically important horticultural fruit crops worldwide. It is critical to gain insights into fruit ripening and softening to improve apple fruit quality and extend shelf life. In this study, forward and reverse suppression subtractive hybridization libraries were generated from 'Taishanzaoxia' apple fruits sampled around the ethylene climacteric to isolate ripening-...
متن کامل